Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis
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چکیده
منابع مشابه
Serotyping of foot-and-mouth disease virus by antigen capture-ELISA using monoclonal antibodies and chicken IgY.
Laboratory detection of specific foot-and-mouth disease virus (FMDV) is routinely carried out by ELISA and RT-PCR. Identification and serotyping of FMDV by ELISA requires polyclonal antibodies raised in rabbits and guinea pigs. The polyclonal antibodies have certain disadvantages such as batch to batch variation, inconsistent yields of antibodies and limited quantity of serum obtained from indi...
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capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...
متن کاملAn ELISA for the quantification of chicken immunoglobulin (IgY) in various liquid media.
The development of an assay for the quantification of chicken immunoglobulin (IgY) and data evaluation is being demonstrated. An enzyme immunoassay was established to perform the quality control of IgY purifications from the egg yolk and to control IgY producing cells in vitro. Each step of the test procedure and possible alternatives were described. The assay was characterized by special test ...
متن کاملThe use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus.
An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV) was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk derived chicken IgY directed against AHSV (serotype 3) was used as the secondary antibody. Since IgY ...
متن کاملDevelopment of an ELISA using anti-idiotypic antibody for diagnosis of opisthorchiasis.
Monoclonal antibody specific for an epitope of cretory-secretory antigen protein of Opisthorchis felineus (Rivolta, 1884) (Trematoda: Opisthorchiidae) with a molecular weight of 28 kDa was used in a sandwich enzyme-linked immunosorbent assay (ELISA) for immobilisation of liver fluke specific antigen to the solid phase. Examination of human sera by this ELISA compared with commercial assays demo...
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ژورنال
عنوان ژورنال: Parasitology International
سال: 2017
ISSN: 1383-5769
DOI: 10.1016/j.parint.2015.10.011